J Perinat Med 30(4):281–286īeckonert O, Monnerjahn J, Bonk U, Leibfritz D (2003) Visualizing metabolic changes in breast-cancer tissue using 1H-NMR spectroscopy and self-organizing maps. Schuller Levis G, Park E, Gregory SM (2006) Is taurine a biomarker? Adv Clin Chem 41:1–21Īerts L, Van Assche FA (2002) Taurine and taurine-deficiency in the perinatal period. Clin Chem 28(8):1758–1761īella DL, Hirschberger LL, Kwon YH, Stipanuk MH (2002) Cysteine metabolism in periportal and perivenous hepatocytes: perivenous cells have greater capacity for glutathione production and taurine synthesis but not for cysteine catabolism. Physiol Rev 73(1):119–147įukuda K, Hirai Y, Yoshida H, Nakajima T, Usui T (1982) Free amino acid content of lymphocytes and granulocytes compared. Sturman JA (1993) Taurine in development. Furthermore, with simple modifications, this method could be applied to other physiological fluids and other types of diseases. Proper clinical validation of these biomarkers can be done using this reliable, fast, and simple method. The parameters affecting separation and quantification were also investigated and optimized. The limit of detection range is 0.18–6 nM and the limit of quantitation ranges from 0.6 to 17.6 nM. Analytes were detected in multiple-reaction monitoring with positive ionization mode. A phenyl-hexyl column was used for the separation at an isocratic condition of 0.2% formic acid in water and 0.2% formic acid in methanol. 15N 2- L-glutamine was used as the internal standard, and creatinine acted as an indicator for urine dilution. In this study, using liquid chromatography–tandem mass spectrometry (LC-MS/MS), a noninvasive method was developed to separate and detect these compounds in urine. However, because of a lack of sensitive and accurate analytical techniques, it is impossible to detect these compounds in urine at low levels. Recently, taurine and several other compounds such as L-phenylalanine and hippuric acid in urine were found to be indicators of bladder cancer. This invasive method causes discomfort and pain in patients. Current urinary bladder cancer diagnosis is commonly based on a biopsy obtained during cystoscopy.
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